A C. Trachomatis Cloning Vector and the Generation of C. Trachomatis Strains Expressing Fluorescent Proteins Under the Control of a C. Trachomatis Promoter

PLoS One. 2013;8(2):e57090. doi: 10.1371/journal.pone.0057090. Epub 2013 Feb 18.

Abstract

Here we describe a versatile cloning vector for conducting genetic experiments in C. trachomatis. We successfully expressed various fluorescent proteins (i.e. GFP, mCherry and CFP) from C. trachomatis regulatory elements (i.e. the promoter and terminator of the incDEFG operon) and showed that the transformed strains produced wild type amounts of infectious particles and recapitulated major features of the C. trachomatis developmental cycle. C. trachomatis strains expressing fluorescent proteins are valuable tools for studying the C. trachomatis developmental cycle. For instance, we show the feasibility of investigating the dynamics of inclusion fusion and interaction with host proteins and organelles by time-lapse video microscopy.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Line
  • Chlamydia trachomatis / genetics*
  • Chlamydia trachomatis / growth & development
  • DNA Replication
  • Gene Expression Regulation, Bacterial*
  • Gene Order
  • Genetic Vectors / genetics*
  • Humans
  • Luminescent Proteins / genetics*
  • Luminescent Proteins / metabolism
  • Operon*

Substances

  • Luminescent Proteins