Molecular analysis of no-on-transient A, a gene required for normal vision in Drosophila

Neuron. 1990 May;4(5):711-23. doi: 10.1016/0896-6273(90)90197-n.

Abstract

Mutation of the Drosophila melanogaster gene no-on-transient A (nonA) results in reduced visual acuity, behavior abnormalities, and an electrophysiological defect for which the mutant is named. We mapped the nonA gene genetically to a 20 kb interval within the 14C1,2 region of the X chromosome, isolated this chromosomal region, and used P element-mediated transformation to delimit the nonA gene to a 9 kb region. Analysis of cDNA clones indicates that this region encodes alternatively spliced transcripts encoding protein products of approximately 77 kd that differ only in their C-terminal 35 amino acids. Analysis of mutations generated in vitro in this transcription unit confirm that these transcripts are the products of the nonA gene.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA / analysis
  • DNA / genetics
  • Drosophila / genetics*
  • Drosophila / physiology
  • Genes / genetics*
  • Genes / physiology
  • Molecular Sequence Data
  • Mutation
  • Proteins / analysis
  • Proteins / genetics*
  • Proteins / physiology
  • Transcription, Genetic
  • Transformation, Genetic
  • Vision, Ocular / physiology*
  • X Chromosome / analysis
  • X Chromosome / ultrastructure

Substances

  • Proteins
  • DNA

Associated data

  • GENBANK/M33496