503unc, a small and muscle-specific zebrafish promoter

Genesis. 2013 Jun;51(6):443-7. doi: 10.1002/dvg.22385. Epub 2013 Mar 26.

Abstract

The muscle-specific UNC-45b assists in the folding of sarcomeric myosin. Analysis of the zebrafish unc-45b upstream region revealed that unc-45b promoter fragments reliably drive GFP expression after germline transmission. The muscle-specific 503-bp minimal promoter 503unc was identified to drive gene expression in the zebrafish musculature. In transgenic Tg(-503unc:GFP) zebrafish, GFP fluorescence was detected in the adaxial cells, their slow fiber descendants, and the fast muscle. At later stages, robust GFP fluorescence is eminent in the cardiac, cranial, fin, and trunk muscle, thereby recapitulating the unc-45b expression pattern. We propose that the 503unc promoter is a small and muscle-specific promoter that drives robust gene expression throughout the zebrafish musculature, making it a valuable tool for the exploration of zebrafish muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism*
  • Molecular Chaperones / genetics*
  • Muscle Development
  • Muscle Proteins
  • Muscle, Skeletal / growth & development
  • Muscle, Skeletal / metabolism*
  • Promoter Regions, Genetic*
  • Sequence Deletion*
  • Zebrafish / genetics*
  • Zebrafish Proteins / genetics*

Substances

  • Molecular Chaperones
  • Muscle Proteins
  • Unc45b protein, zebrafish
  • Zebrafish Proteins
  • Green Fluorescent Proteins