Abstract
The native HIV-1 Env complex consists of a gp120/gp41 trimer, but surface plasmon resonance (SPR)-directed binding studies for gp120-binding agents were almost exclusively performed on monomeric gp120. SPR-directed binding kinetics of monomeric gp120 and trimeric gp140 were investigated for a broad variety of envelope (Env)-binding agents. Similar kinetics for carbohydrate-binding agents (CBAs), the antibody 2G12 and sCD4 were observed, irrespective of the oligomeric state of gp120 that either contain the native mixture of complex and high-mannose N-glycans or that contain exclusively oligomannose N-glycans. The generally comparable kinetic properties of CBA, 2G12 and sCD4 binding to monomeric gp120 and trimeric gp140 indicate that monomeric gp120 is a good surrogate molecule for native HIV-1 Env trimer to investigate the binding affinities of Env-binding compounds.
Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / metabolism
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Bacterial Proteins / metabolism
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Binding, Competitive
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Blotting, Western
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Broadly Neutralizing Antibodies
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HEK293 Cells
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HIV Antibodies
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HIV Envelope Protein gp120 / chemistry
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HIV Envelope Protein gp120 / genetics
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HIV Envelope Protein gp120 / metabolism*
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HIV Envelope Protein gp41 / chemistry
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HIV Envelope Protein gp41 / genetics
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HIV Envelope Protein gp41 / metabolism*
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HIV-1 / genetics
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HIV-1 / metabolism*
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Humans
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Kinetics
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Mannose / metabolism
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Plant Lectins / metabolism
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Polysaccharides / metabolism
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Protein Binding
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Protein Multimerization
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Surface Plasmon Resonance
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env Gene Products, Human Immunodeficiency Virus / chemistry
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env Gene Products, Human Immunodeficiency Virus / genetics
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env Gene Products, Human Immunodeficiency Virus / metabolism*
Substances
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2G12 monoclonal antibody
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Antibodies, Monoclonal
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Bacterial Proteins
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Broadly Neutralizing Antibodies
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HIV Antibodies
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HIV Envelope Protein gp120
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HIV Envelope Protein gp41
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Plant Lectins
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Polysaccharides
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actinohivin protein, actinomycete
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env Gene Products, Human Immunodeficiency Virus
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gp140 envelope protein, Human immunodeficiency virus 1
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Mannose