Low pH D-xylonate production with Pichia kudriavzevii

Bioresour Technol. 2013 Apr;133:555-62. doi: 10.1016/j.biortech.2013.01.157. Epub 2013 Feb 7.

Abstract

D-xylonic acid is one of the top 30 most desirable chemicals to be derived from biomass sugars identified by the US Department of Energy, being applicable as a non-food substitute for D-gluconic acid and as a platform chemical. We engineered the non-conventional yeast Pichia kudriavzevii VTT C-79090T to express a D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant P. kudriavzevii strain produced up to 171 g L(-1) of D-xylonate from 171 g L(-1) D-xylose at a rate of 1.4 g L(-1) h(-1) and yield of 1.0 g [g substrate consumed](-1), which was comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain was also remarkable at low pH, producing 146 g L(-1) D-xylonate at 1.2 g L(-1) h(-1) at pH 3.0. This is the best low pH production reported for D-xylonate. These results encourage further development towards industrial scale production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomass
  • Glucose / pharmacology
  • Hydrogen-Ion Concentration / drug effects
  • Intracellular Space / drug effects
  • Intracellular Space / metabolism
  • Kluyveromyces / drug effects
  • Kluyveromyces / growth & development
  • Kluyveromyces / metabolism
  • Pichia / drug effects
  • Pichia / growth & development
  • Pichia / metabolism*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism
  • Sugar Acids / metabolism*
  • Xylitol / metabolism
  • Xylose / biosynthesis*

Substances

  • Sugar Acids
  • Xylose
  • Glucose
  • Xylitol