Ultrahigh accuracy imaging modality for super-localization microscopy

Nat Methods. 2013 Apr;10(4):335-8. doi: 10.1038/nmeth.2396. Epub 2013 Mar 3.


Super-localization microscopy encompasses techniques that depend on the accurate localization of individual molecules from generally low-light images. The obtainable localization accuracies, however, are ultimately limited by the image detector's pixelation and noise. We present the ultrahigh accuracy imaging modality (UAIM), which allows users to obtain accuracies approaching the accuracy that is achievable only in the absence of detector pixelation and noise, and which we found can experimentally provide a >200% accuracy improvement over conventional low-light imaging.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Equipment Design
  • Fluorescence
  • Likelihood Functions
  • Microscopy / methods*
  • Photons
  • Protein Transport / physiology*
  • Signal Processing, Computer-Assisted / instrumentation
  • Stochastic Processes