[Determination of doping in human urine by gas chromatography-high resolution mass spectrometry]

Yao Xue Xue Bao. 2012 Dec;47(12):1667-70.
[Article in Chinese]

Abstract

A method was evaluated for determination of twenty-one doping (including nandrolone, boldenone and methandienone) in human urine by gas chromatography-high resolution mass spectrometry. Samples were prepared by liquid-liquid extraction, concentrated, TMS derivatization and limit of detection at ng x mL(-1) by MID/GC/HRMS. According to the code of the World Anti-Doping Agency (WADA), precision and recoveries of the procedure were evaluated by replicate analysis (n = 6), the recoveries in the range of 66%-103%, with the RSD below 10.0%. The precision within the day of the method with three different concentrations was also determined RSD were less than 9.5%, 10.0% and 9.7%.

Publication types

  • English Abstract

MeSH terms

  • Anabolic Agents / urine*
  • Doping in Sports*
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Methandrostenolone / urine*
  • Nandrolone / urine*
  • Substance Abuse Detection / methods
  • Testosterone / analogs & derivatives*
  • Testosterone / urine

Substances

  • Anabolic Agents
  • Testosterone
  • boldenone
  • Nandrolone
  • Methandrostenolone