Autofluorescence generation and elimination: a lesson from glutaraldehyde

Chem Commun (Camb). 2013 Apr 14;49(29):3028-30. doi: 10.1039/c3cc40799c.

Abstract

Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible to near-IR emitters. A model indicated that ethylenediamine and a secondary amine in the molecule were key components for the formation of emissive species. The mechanism enables us to control the generation and elimination of autofluorescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines / chemistry
  • Amino Acid Sequence
  • Carbocyanines / chemistry
  • Cell Line, Tumor
  • Ethylenediamines / chemistry
  • Glutaral / chemistry*
  • Humans
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Peptides / chemistry*
  • Peptides / metabolism
  • Proteins / chemistry*
  • Proteins / metabolism
  • Spectrophotometry

Substances

  • Amines
  • Carbocyanines
  • Ethylenediamines
  • Peptides
  • Proteins
  • cyanine dye 3
  • ethylenediamine
  • Glutaral