Abstract
Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible to near-IR emitters. A model indicated that ethylenediamine and a secondary amine in the molecule were key components for the formation of emissive species. The mechanism enables us to control the generation and elimination of autofluorescence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amines / chemistry
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Amino Acid Sequence
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Carbocyanines / chemistry
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Cell Line, Tumor
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Ethylenediamines / chemistry
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Glutaral / chemistry*
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Humans
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Hydrogen-Ion Concentration
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Molecular Sequence Data
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Peptides / chemistry*
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Peptides / metabolism
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Proteins / chemistry*
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Proteins / metabolism
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Spectrophotometry
Substances
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Amines
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Carbocyanines
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Ethylenediamines
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Peptides
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Proteins
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cyanine dye 3
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ethylenediamine
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Glutaral