The anti-wrinkle efficacy of Argireline

J Cosmet Laser Ther. 2013 Aug;15(4):237-41. doi: 10.3109/14764172.2013.769273. Epub 2013 Mar 6.


Background: Argireline, a synthetic peptide, which is patterned from the N-terminal end of the protein SNAP-25, can both reduce the degree of existing facial wrinkles and demonstrate effectively against their development. In our past studies, we found out that Argireline had a significant anti-wrinkle effect in Chinese subjects and that it was safe and well tolerated.

Objective: To observe the effect of Argireline on histological changes in the skin in the aged mice induced by D-galactose.

Methods: Argireline was applied to the aged mice twice daily for 6 weeks. The histological changes in skin tissue were evaluated using hematoxylin-eosin (HE) and picrosirius-polarization (PSP) stains. The amount of type I and of type III collagen fibers were also semi-quantitatively compared using software Image-ProPlus.

Results: There was an improvement in the histological structure of skin tissue in the aged mice; the amount of type I collagen fibers increased (P < 0.01), while that of type III collagen fibers decreased (P < 0.05).

Conclusions: This study revealed that Argireline could improve the histological structure of skin tissue and rejuvenate the aging skin.

MeSH terms

  • Animals
  • Collagen Type I / drug effects
  • Collagen Type I / metabolism
  • Collagen Type III / drug effects
  • Collagen Type III / metabolism
  • Mice
  • Oligopeptides / pharmacology*
  • Skin / anatomy & histology
  • Skin Aging / drug effects*
  • Staining and Labeling


  • Collagen Type I
  • Collagen Type III
  • Oligopeptides
  • acetyl-glutamyl-glutamyl-methionyl-glutaminyl-arginyl-argininamide