Inducible cytosolic enzyme activity for the production of nitrogen oxides from L-arginine in hepatocytes

Biochem Biophys Res Commun. 1990 May 16;168(3):1034-40. doi: 10.1016/0006-291x(90)91133-d.


The in vivo conditions needed for the induction of nitrogen oxide synthesis by hepatocytes were determined. Hepatocytes obtained from rats injected with killed Corynebacterium parvum spontaneously produced NO2(-)+NO3- in culture and were found to contain cytosolic enzyme activity for nitrogen oxide synthesis. The enzyme activity required both L-arginine and NADPH, and was not found in hepatocytes obtained from normal rats or rats injected with lipopolysaccharide (LPS) alone. In contrast, nonparenchymal cells were stimulated to synthesize NO2(-)+NO3- by LPS. These results show the presence of inducible cytosolic enzyme activity for nitrogen oxide synthesis in hepatocytes, which is distinct from nonparenchymal cell NO. synthesis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arginine / metabolism*
  • Cells, Cultured
  • Cytosol / enzymology*
  • Enzyme Induction* / drug effects
  • Ligases / biosynthesis*
  • Lipopolysaccharides / pharmacology
  • Liver / cytology
  • Liver / enzymology
  • Liver / metabolism*
  • Male
  • NADP / pharmacology
  • Nitrogen Oxides / metabolism*
  • Rats
  • Rats, Inbred Strains


  • Lipopolysaccharides
  • Nitrogen Oxides
  • NADP
  • Arginine
  • Ligases