Preparation of poly(hydroxyethyl methacrylate) cryogels containing L-histidine for insulin recognition

Colloids Surf B Biointerfaces. 2013 Jul 1:107:84-9. doi: 10.1016/j.colsurfb.2013.01.075. Epub 2013 Feb 10.

Abstract

In the present study, affinity adsorption technique was studied for insulin adsorption. Firstly, insulin-imprinted supermacroporous cryogel was prepared for the insulin adsorption. N-methacryloyl-(L)-histidine methyl ester (MAH) was chosen as the monomer. Insulin was complexed with MAH, and insulin-imprinted p(HEMA-MAH) [insulin-(MIP)] cryogel was prepared by free radical polymerization with 2-hydroxyethyl methacrylate (HEMA), N,N,N',N'-tetramethylethylenediamine (TEMED) and ammonium persulfate (APS) in an ice bath. Then, insulin was removed from the cryogel by using 0.1 M glycine-HCl buffer (pH: 3.5). The characterization of the cryogel was carried out by using scanning electron microscopy (SEM) and swelling test. The equilibrium swelling ratios of the cryogels were found to be 8.56±0.42 g H2O/g polymer for p(HEMA) and 7.20±0.36 g H2O/g polymer for insulin-p(HEMA-MAH). Insulin adsorption experiments were performed under different conditions, such as flow rate, medium pH, initial insulin concentration and ionic strength. It was observed that insulin could be repeatedly adsorbed and desorbed with MIP cryogel without any significant decrease in the adsorption capacity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Aprotinin / chemistry
  • Chromatography, High Pressure Liquid
  • Cryogels / chemical synthesis*
  • Cryogels / chemistry
  • Histidine / chemistry*
  • Hydrogen-Ion Concentration
  • Insulin / analysis*
  • Kinetics
  • Microscopy, Electron, Scanning
  • Molecular Imprinting
  • Osmolar Concentration
  • Polyhydroxyethyl Methacrylate / chemical synthesis*
  • Polyhydroxyethyl Methacrylate / chemistry
  • Recycling
  • Rheology
  • Temperature

Substances

  • Cryogels
  • Insulin
  • Polyhydroxyethyl Methacrylate
  • Histidine
  • Aprotinin