Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae

Mol Biosyst. 2013 Jun;9(6):1351-9. doi: 10.1039/c3mb25516f. Epub 2013 Mar 7.


A genome-wide screen of a yeast non-essential gene-deletion library was used to identify sick phenotypes due to oxygen deprivation. The screen provided a manageable list of 384 potentially novel as well as known oxygen responding (anoxia-survival) genes. The gene-deletion mutants were further assayed for sensitivity to ferrozine and cobalt to obtain a subset of 34 oxygen-responsive candidate genes including the known hypoxic gene activator, MGA2. With each mutant in this subset a plasmid based β-galactosidase assay was performed using the anoxic-inducible promoter from OLE1 gene, and 17 gene deletions were identified that inhibit induction under anaerobic conditions. Genetic interaction analysis for one of these mutants, the RNase-encoding POP2 gene, revealed synthetic sick interactions with a number of genes involved in oxygen sensing and response. Knockdown experiments for CNOT8, human homolog of POP2, reduced cell survival under low oxygen condition suggesting a similar function in human cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Hypoxia
  • Cell Line
  • Cell Survival / genetics
  • Cobalt / pharmacology
  • Fatty Acid Desaturases / genetics
  • Ferrozine / pharmacology
  • Gene Deletion
  • Gene Expression Profiling
  • Gene Expression Regulation, Fungal
  • Humans
  • Iron Chelating Agents / metabolism
  • Membrane Proteins / genetics
  • Oxygen / metabolism
  • Promoter Regions, Genetic
  • Ribonucleases / genetics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Stearoyl-CoA Desaturase
  • Trace Elements / metabolism
  • Transcription Factors / genetics
  • Transcriptional Activation
  • beta-Galactosidase / genetics


  • CNOT8 protein, human
  • Iron Chelating Agents
  • MGA2 protein, S cerevisiae
  • Membrane Proteins
  • Saccharomyces cerevisiae Proteins
  • Trace Elements
  • Transcription Factors
  • Ferrozine
  • Cobalt
  • Fatty Acid Desaturases
  • Stearoyl-CoA Desaturase
  • delta-9 fatty acid desaturase
  • Ribonucleases
  • POP2 protein, S cerevisiae
  • beta-Galactosidase
  • Oxygen