Abstract
We have shown that Dicer processes 7SL RNA into different fragments ranging from ∼20 to more than 200 nucleotides. Here we addressed the molecular functions of these 7SL RNA fragments and found that some of them functioned as dominant-negative regulators of the full-length 7SL RNA, interfering with signal recognition particle (SRP) complex formation. Transfection of these 7SL RNA fragments inhibited the expression of cell surface glycoproteins, the targeting of a reporter protein to the endoplasmic reticulum, and the secretion of secreted alkaline phosphatase. These results suggest that some Dicer-processed 7SL RNA fragments interfered with SRP-mediated protein targeting. Moreover, we showed that Dicer knockdown enhanced SRP-mediated protein targeting and that transfection of a mixture of the 7SL RNA fragments partially restored this effect. Our data indicate that Dicer can fine-tune the efficiency of SRP-mediated protein targeting via processing a proportion of 7SL RNA into fragments of different lengths.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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DEAD-box RNA Helicases / genetics*
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Gene Expression
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Gene Knockdown Techniques
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Humans
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Multiprotein Complexes / metabolism
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Protein Transport
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RNA, Small Cytoplasmic / genetics
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RNA, Small Cytoplasmic / metabolism
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Ribonuclease III / genetics*
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Signal Recognition Particle / genetics
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Signal Recognition Particle / metabolism*
Substances
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7SL RNA
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Multiprotein Complexes
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RNA, Small Cytoplasmic
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Signal Recognition Particle
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DICER1 protein, human
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Ribonuclease III
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DEAD-box RNA Helicases
Grants and funding
This work was supported by National Natural Science Foundation of China (30971612, 81171967, 31271383 and 30700708 to KFT); Zhejiang Provincial Natural Science Foundation (R2110503 to KFT, Y2101210 to JW); Wenzhou Science and Technology Foundation (Y20100202 to KFT, Y20100203 to JW) and the key project of Chongqing Medical Science Foundation (09-1-17 to KFT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.