γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells

PLoS One. 2013;8(2):e57779. doi: 10.1371/journal.pone.0057779. Epub 2013 Feb 28.

Abstract

Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Caspase 3 / metabolism
  • Cell Death / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Chromans / pharmacology*
  • Colonic Neoplasms / pathology*
  • Enzyme Activation / drug effects
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Signal Transduction / drug effects
  • Vacuoles / drug effects
  • Vacuoles / metabolism
  • Vitamin E / analogs & derivatives*
  • Vitamin E / pharmacology
  • Wnt Proteins / genetics
  • Wnt Proteins / metabolism

Substances

  • Antineoplastic Agents
  • Chromans
  • Wnt Proteins
  • Vitamin E
  • plastochromanol 8
  • Caspase 3

Grant support

This research was supported by the Chinese National Natural Science Foundation (No. 30771801) and the Science and Chinese Technology Foundation of Tianjin Health Bureau (No. 07KZ73). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.