RNA-guided genome editing à la carte

Cell Res. 2013 Jun;23(6):733-4. doi: 10.1038/cr.2013.39. Epub 2013 Mar 12.

Abstract

Two recent papers in Science illustrate how the prokaryotic CRISPR-Cas immune system machinery, which typically targets invasive genetic elements such as viruses and plasmids, can be converted into a sophisticated molecular tool for next-generation human genome editing. The versatile Cas9 RNA-guided endonuclease can be readily reprogrammed using customizable small RNAs for sequence-specific single- or double-stranded DNA cleavage.

MeSH terms

  • CRISPR-Cas Systems / genetics*
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
  • DNA Restriction Enzymes
  • Endonucleases
  • Genetic Engineering
  • Humans
  • Plasmids / genetics
  • RNA / genetics
  • RNA / metabolism
  • RNA Editing / genetics*
  • RNA, Guide / genetics*
  • RNA, Small Interfering / genetics
  • Viruses / genetics

Substances

  • RNA, Guide
  • RNA, Small Interfering
  • RNA
  • Endonucleases
  • DNA Restriction Enzymes