RNA-Seq: revelation of the messengers

Trends Plant Sci. 2013 Apr;18(4):175-9. doi: 10.1016/j.tplants.2013.02.001. Epub 2013 Mar 5.


Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the technology of choice for whole-transcriptome studies. However, the bioinformatics skills required for RNA-Seq data analysis often pose a significant hurdle for many biologists. Here, we put forward the concepts and considerations that are critical for RNA-Seq data analysis and provide a generic tutorial with example data that outlines the whole pipeline from next-generation sequencing output to quantification of differential gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Computational Biology
  • DNA, Complementary / genetics
  • Gene Expression Profiling / methods*
  • Gene Library
  • High-Throughput Nucleotide Sequencing / methods*
  • RNA, Messenger / genetics*
  • Sequence Analysis, RNA / methods*
  • Transcriptome


  • DNA, Complementary
  • RNA, Messenger