Anxiolytic effects of Julibroside C1 isolated from Albizzia julibrissin in mice

Prog Neuropsychopharmacol Biol Psychiatry. 2013 Jul 1;44:184-92. doi: 10.1016/j.pnpbp.2013.02.012. Epub 2013 Feb 26.

Abstract

Julibroside C1 is a saponin-containing compound isolated from Albizzia julibrissin Durazz. In this study, we investigated the putative anxiolytic effects of Julibroside C1 using the elevated plus maze (EPM) in mice. Julibroside C1 at doses of 0.5 and 1 mg/kg significantly increased the time spent in the open arms and the number of entries into the open arms of the EPM compared to the control group. Moreover, the anxiolytic-like effects of Julibroside C1 (0.5 mg/kg) were blocked by WAY-100635 (5-HT1A receptor antagonist), bicuculline (GABA(A) receptor antagonist), and flumazenil (antagonist of the GABA(A) receptor benzodiazepine site). However, Julibroside C1 did not change locomotor activity or induce myorelaxant effects. We used quantitative receptor autoradiography to investigate the effects of Julibroside C1 on alterations in mouse brain receptors. After acute treatment with Julibroside C1 (0.5 mg/kg), [(3)H]-8-OH-DPAT binding was significantly decreased in the CA1 region of the hippocampus and [(3)H]-flunitrazepam binding was decreased remarkably in the cingulate cortex region. However, [(3)H]-muscimol binding did not show a significant change in any brain region. Taken together, our findings suggest that Julibroside C1 shows anxiolytic-like effects, which might be mediated by the 5-HT1A and GABA(A)-benzodiazepine receptor systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2-(di-n-propylamino)tetralin / pharmacokinetics
  • Albizzia / chemistry*
  • Analysis of Variance
  • Animals
  • Anti-Anxiety Agents / pharmacology*
  • Autoradiography
  • Brain / diagnostic imaging
  • Brain / drug effects
  • Dose-Response Relationship, Drug
  • Flunitrazepam / pharmacokinetics
  • Male
  • Maze Learning / drug effects*
  • Mice
  • Mice, Inbred ICR
  • Motor Activity / drug effects*
  • Muscimol / pharmacokinetics
  • Plant Preparations / chemistry
  • Plant Preparations / pharmacology
  • Protein Binding / drug effects
  • Radionuclide Imaging
  • Saponins / chemistry
  • Saponins / pharmacology*
  • Triterpenes / chemistry
  • Triterpenes / pharmacology*
  • Tritium / pharmacokinetics

Substances

  • Anti-Anxiety Agents
  • Plant Preparations
  • Saponins
  • Triterpenes
  • julibroside C1
  • Tritium
  • Muscimol
  • Flunitrazepam
  • 8-Hydroxy-2-(di-n-propylamino)tetralin