Resazurin-based assay for screening bacteria for radiation sensitivity

Springerplus. 2013 Dec;2(1):55. doi: 10.1186/2193-1801-2-55. Epub 2013 Feb 16.


We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our colorimetric method was as accurate as the standard method and was superior in terms of savings in supplies and man-hours.

Keywords: Escherichia coli; High throughput; Radiation-sensitivity assay; Resazurin; UV radiation; X-radiation.