Degradome sequencing reveals an endogenous microRNA target in C. elegans

FEBS Lett. 2013 Apr 2;587(7):964-9. doi: 10.1016/j.febslet.2013.02.029. Epub 2013 Feb 26.

Abstract

Caenorhabditis elegans microRNAs (miRNAs) bind to partially complementary sequences in the 3' untranslated region of target mRNAs, resulting in translational repression through mRNA destabilization. High-throughput sequencing of RNA cleavage fragments was performed to directly detect miRNA-directed cleavage targets in adult stage C. elegans. From this analysis, we found that miR-249 directed the cleavage of the ZK637.6 transcript with extensive and evolutionarily conserved complementarity in nematode. In addition, expression of the ZK637.6 transcript was strongly dependent on the expression of miR-249. These findings may lead to a better understanding of miRNA-mediated gene regulation in nematodes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Animals
  • Arsenite Transporting ATPases / genetics*
  • Arsenite Transporting ATPases / metabolism
  • Base Sequence
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins / genetics*
  • Caenorhabditis elegans Proteins / metabolism
  • Gene Expression Profiling
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Molecular Sequence Data
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • RNA, Helminth / genetics
  • RNA, Helminth / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, RNA / methods*
  • Sequence Homology, Nucleic Acid

Substances

  • 3' Untranslated Regions
  • Caenorhabditis elegans Proteins
  • MIRN249 microRNA, C elegans
  • MicroRNAs
  • RNA, Helminth
  • ASNA-1 protein, C elegans
  • Arsenite Transporting ATPases

Associated data

  • GEO/GSE44336