Serum- and glucocorticoid-regulated kinase 1 is required for nuclear export of the ribonucleoprotein of influenza A virus

J Virol. 2013 May;87(10):6020-6. doi: 10.1128/JVI.01258-12. Epub 2013 Mar 13.

Abstract

We previously performed a small interfering RNA (siRNA) screen and identified serum- and glucocorticoid-regulated kinase 1 (SGK1) as a host factor required for influenza A virus replication. However, the role of SGK1 in the influenza viral life cycle has never been examined. In this study, we demonstrate that SGK1 is required for optimal replication of influenza virus, using the SGK1 inhibitor GSK 650394 and SGK1-specific siRNAs. We also demonstrate that SGK1 is required for viral ribonucleoprotein nuclear export.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus*
  • Benzoates / metabolism
  • Bridged Bicyclo Compounds, Heterocyclic / metabolism
  • Cell Line
  • Gene Knockdown Techniques
  • Host-Pathogen Interactions*
  • Humans
  • Immediate-Early Proteins / metabolism*
  • Influenza A virus / physiology*
  • Protein-Serine-Threonine Kinases / metabolism*
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Ribonucleoproteins / metabolism*
  • Virus Replication*

Substances

  • Benzoates
  • Bridged Bicyclo Compounds, Heterocyclic
  • Immediate-Early Proteins
  • RNA, Small Interfering
  • Ribonucleoproteins
  • 2-cyclopentyl-4-(5-phenyl-1H-pyrrolo(2,3-b)pyridin-3-yl)-benzoic acid
  • Protein-Serine-Threonine Kinases
  • serum-glucocorticoid regulated kinase