RNA-based fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP

J Am Chem Soc. 2013 Apr 3;135(13):4906-9. doi: 10.1021/ja311960g. Epub 2013 Mar 21.


Cyclic dinucleotides are an important class of signaling molecules that regulate a wide variety of pathogenic responses in bacteria, but tools for monitoring their regulation in vivo are lacking. We have designed RNA-based fluorescent biosensors for cyclic di-GMP and cyclic AMP-GMP by fusing the Spinach aptamer to variants of a natural GEMM-I riboswitch. In live cell imaging experiments, these biosensors demonstrate fluorescence turn-on in response to cyclic dinucleotides, and they were used to confirm in vivo production of cyclic AMP-GMP by the enzyme DncV.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Aptamers, Nucleotide / genetics
  • Biosensing Techniques / methods*
  • Cyclic AMP / chemistry*
  • Cyclic AMP / genetics
  • Cyclic GMP / analogs & derivatives*
  • Cyclic GMP / chemistry*
  • Cyclic GMP / genetics
  • Fluorescence
  • Molecular Imaging / methods*
  • RNA / chemistry*
  • Spinacia oleracea / genetics


  • Aptamers, Nucleotide
  • bis(3',5')-cyclic diguanylic acid
  • RNA
  • Cyclic AMP
  • Cyclic GMP