Integration of human immunodeficiency virus type 1 DNA in vitro

Proc Natl Acad Sci U S A. 1990 Jun;87(11):4164-8. doi: 10.1073/pnas.87.11.4164.

Abstract

A highly efficient cell-free system for the integration of human immunodeficiency virus type 1 DNA is described. Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection. The linear viral DNA molecules present in cytoplasmic extracts are capable of integrating into heterologous DNA targets in vitro. The viral DNA resides in a high molecular weight nucleoprotein structure that can be separated from the bulk of cellular protein and nucleic acid without a detectable decrease in the ability to integrate in vitro.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Southern
  • Cell Line
  • Cell-Free System
  • Cytoplasm / microbiology
  • DNA / metabolism*
  • DNA, Viral / biosynthesis
  • DNA, Viral / metabolism*
  • Deoxyribonucleoproteins / metabolism
  • HIV-1 / genetics*
  • Humans
  • In Vitro Techniques
  • Recombination, Genetic
  • Time Factors
  • Virus Replication

Substances

  • DNA, Viral
  • Deoxyribonucleoproteins
  • DNA