Identification and verification of microRNAs by high-throughput sequencing

Methods Mol Biol. 2013:983:125-38. doi: 10.1007/978-1-62703-302-2_7.

Abstract

High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern / methods
  • Computational Biology
  • Dictyostelium / genetics
  • Electrophoresis, Polyacrylamide Gel
  • High-Throughput Nucleotide Sequencing / methods*
  • MicroRNAs / genetics*
  • MicroRNAs / isolation & purification
  • RNA, Protozoan / genetics*
  • RNA, Protozoan / isolation & purification
  • Sequence Analysis, RNA / methods

Substances

  • MicroRNAs
  • RNA, Protozoan