Exon-level expression profiling of ocular tissues

Exp Eye Res. 2013 Jun;111:105-11. doi: 10.1016/j.exer.2013.03.004. Epub 2013 Mar 14.

Abstract

The normal gene expression profiles of the tissues in the eye are a valuable resource for considering genes likely to be involved with disease processes. We profiled gene expression in ten ocular tissues from human donor eyes using Affymetrix Human Exon 1.0 ST arrays. Ten different tissues were obtained from six different individuals and RNA was pooled. The tissues included: retina, optic nerve head (ONH), optic nerve (ON), ciliary body (CB), trabecular meshwork (TM), sclera, lens, cornea, choroid/retinal pigment epithelium (RPE) and iris. Expression values were compared with publically available Expressed Sequence Tag (EST) and RNA-sequencing resources. Known tissue-specific genes were examined and they demonstrated correspondence of expression with the representative ocular tissues. The estimated gene and exon level abundances are available online at the Ocular Tissue Database.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Choroid / physiology
  • Ciliary Body / physiology
  • Exons / genetics*
  • Eye Banks
  • Humans
  • Lens, Crystalline / physiology
  • Ocular Physiological Phenomena / genetics*
  • Oligonucleotide Array Sequence Analysis*
  • Optic Disk / physiology
  • Retina / physiology
  • Sclera / physiology
  • Trabecular Meshwork / physiology
  • Transcriptome*