Gene expression levels of S100 protein family in blood cells are associated with insulin resistance and inflammation (Peripheral blood S100 mRNAs and metabolic syndrome)

Biochem Biophys Res Commun. 2013 Apr 19;433(4):450-5. doi: 10.1016/j.bbrc.2013.02.096. Epub 2013 Mar 15.


Objective: Visceral fat obesity is located upstream of metabolic syndrome and atherosclerotic diseases. Accumulating evidences indicate that several immunocytes including macrophages infiltrate into adipose tissue and induce chronic low-grade inflammation. We recently analyzed the association between visceral fat adiposity and the gene expression profile in peripheral blood cells in human subjects and demonstrated the close relationship of visceral fat adiposity and disturbance of circadian rhythm in peripheral blood cells. In a series of studies, we herein investigated the association of visceral fat adiposity and mRNA levels relating to inflammatory genes in peripheral blood cells.

Approach and results: Microarray analysis was performed in peripheral blood cells from 28 obese subjects. Reverse transcription-polymerase chain reaction (RT-PCR) was conducted by using blood cells from 57 obese subjects. Obesity was defined as body mass index (BMI) greater than 25 kg/m2 according to the Japanese criteria. Gene expression profile analysis was carried out with Agilent whole human genome 4×44K oligo-DNA microarray. Gene ontology (GO) analysis showed that 14 genes were significantly associated with visceral fat adiposity among 239 genes relating to inflammation. Among 14 genes, RT-PCR demonstrated that S100A8, S100A9, and S100A12 positively correlated with visceral fat adiposity in 57 subjects. Stepwise multiple regression analysis showed that S100A8 and S100A12 mRNA levels were closely associated with HOMA-IR and S100A9 mRNA was significantly related to adiponectin and CRP.

Conclusions: Peripheral blood mRNA levels of S100 family were closely associated with insulin resistance and inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiponectin / blood
  • Adiposity
  • Asian Continental Ancestry Group
  • Blood Cells / pathology
  • Body Mass Index
  • C-Reactive Protein / analysis
  • Calgranulin A / blood
  • Calgranulin A / genetics
  • Calgranulin B / blood
  • Calgranulin B / genetics
  • Gene Expression Regulation
  • Genetic Association Studies
  • Genome, Human
  • Humans
  • Inflammation / genetics
  • Inflammation / pathology*
  • Insulin Resistance*
  • Intra-Abdominal Fat / pathology
  • Metabolic Syndrome / genetics
  • Metabolic Syndrome / pathology*
  • Obesity / genetics
  • Obesity / pathology*
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger / blood*
  • RNA, Messenger / genetics
  • Regression Analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • S100 Proteins / blood*
  • S100 Proteins / genetics
  • S100A12 Protein
  • Transcriptome


  • ADIPOQ protein, human
  • Adiponectin
  • Calgranulin A
  • Calgranulin B
  • RNA, Messenger
  • S100 Proteins
  • S100A12 Protein
  • S100A12 protein, human
  • C-Reactive Protein