TGF-beta 1 Binding Protein: A Component of the Large Latent Complex of TGF-beta 1 With Multiple Repeat Sequences

Cell. 1990 Jun 15;61(6):1051-61. doi: 10.1016/0092-8674(90)90069-q.

Abstract

TGF-beta occurs in a latent complex of high Mr. We report the cDNA cloning and an initial structural and functional characterization of a component of the large latent TGF-beta 1 complex, denoted TGF-beta 1 binding protein (TGF-beta 1-BP). Most of the sequence of fibroblast TGF-beta 1-BP is made up of cysteine-rich repeats of two different kinds; there are 16 EGF-like repeats and three repeats with a distant resemblance to EGF, but of a distinct type hitherto not found in any other protein. beta-hydroxylated asparagine residues were identified in two of the EGF-like repeats. TGF-beta 1-BP purified from human platelets is considerably smaller than the fibroblast form (125-160 kd vs. 170-190 kd), suggesting that there is alternative splicing of the TGF-beta 1-BP gene or that TGF-beta 1-BP undergoes cell-specific proteolysis. TGF-beta 1-BP was found not to bind and inactive TGF-beta 1; its role in the latent complex is discussed.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Asparagine / analogs & derivatives
  • Asparagine / analysis
  • Base Sequence
  • Blood Platelets / metabolism
  • Blotting, Northern
  • Carrier Proteins / genetics*
  • Carrier Proteins / isolation & purification
  • Cell Line
  • Cloning, Molecular
  • DNA / blood
  • DNA / genetics
  • DNA / isolation & purification
  • Gene Expression
  • Humans
  • Intracellular Signaling Peptides and Proteins*
  • Latent TGF-beta Binding Proteins
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Peptide Fragments / isolation & purification
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Repetitive Sequences, Nucleic Acid
  • Transforming Growth Factors / genetics*

Substances

  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Latent TGF-beta Binding Proteins
  • Peptide Fragments
  • RNA, Messenger
  • 3-hydroxyasparagine
  • Asparagine
  • Transforming Growth Factors
  • DNA

Associated data

  • GENBANK/M34057