UPLC-Q-TOF-MS/MS fingerprinting of Traditional Chinese Formula SiJunZiTang

Yanyan Wang; Shan He; Xiaochen Cheng; Yuxin Lu; Yunpeng Zou; Qinglin Zhang

doi:10.1016/j.jpba.2013.02.021

UPLC-Q-TOF-MS/MS fingerprinting of Traditional Chinese Formula SiJunZiTang

J Pharm Biomed Anal. 2013 Jun:80:24-33. doi: 10.1016/j.jpba.2013.02.021. Epub 2013 Feb 27.

Authors

Yanyan Wang¹, Shan He, Xiaochen Cheng, Yuxin Lu, Yunpeng Zou, Qinglin Zhang

Affiliation

¹ School of Pharmacy, Guangxi Medical University, Shuangyong Road 22, Nanning 530021, PR China; Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing, PR China.

PMID: 23511229
DOI: 10.1016/j.jpba.2013.02.021

Abstract

SiJunZiTang (SJZT), a classic Traditional Chinese Formula (TCMF) consisting of four herbs, Radix Ginseng, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis, has been demonstrated to show protective effects on intestine and stomach injure. The chromatographic quality control is needed. UPLC-Q-TOF-MS is a rapid and efficient technique for analysis of complex sample in combination with UPLC and tandem mass spectrometry (MS/MS). In this paper, UPLC-MS fingerprinting of SJZT was developed. As a result, 66 compounds including ginsenosides, flavonoids, triterpenoid and coumarins were detected, 58 of them were tentatively identified. The major constituents of SJZT were ginsenosides and flavonoids that coming from Radix Ginseng and Glycyrrhiza uralensis.

MeSH terms

Chromatography, Liquid / methods*
Drugs, Chinese Herbal / analysis*
Drugs, Chinese Herbal / chemistry
Medicine, Chinese Traditional / methods
Quality Control
Tandem Mass Spectrometry / methods*
Time Factors

Substances

Drugs, Chinese Herbal