A Kluyvera sp. was recovered from the urine of a previously healthy 5-year-old child with clinical and laboratory evidence of acute pyelonephritis. This pathogen is usually a saprophyte and even when recovered in humans it is ordinarily considered an opportunistic pathogen. Only two other cases of Kluyvera infection have been described in immunocompetent individuals. The case presented further supports the finding that this bacterium can cause severe disease even in previously healthy, immunocompetent children.