Amplification and overexpression of the epidermal growth factor receptor gene in human renal-cell carcinoma

Int J Cancer. 1990 Jun 15;45(6):1018-21. doi: 10.1002/ijc.2910450606.

Abstract

We examined 22 cases of renal-cell carcinoma (RCC) for structural alterations of the epidermal growth factor receptor (EGFR) gene and found gene amplification in one case of high-stage-high-grade RCC. Dot blot analysis of the total RNA from tumorous and normal kidney tissues revealed overexpression of the EGFR gene in 12 of 20 (60%) cases of RCC. The highest expression was observed in the gene-amplified case. No correlation was observed between the level of EGFR mRNA and tumor stage or grade. Northern blot analysis revealed normal 10- and 5.6-kb EGFR mRNA bands in RCC. Our data indicate that gene amplification of the EGFR gene is one of the molecular mechanisms of its overexpression in a subset of RCCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Blotting, Southern
  • Carcinoma, Renal Cell / genetics*
  • DNA Probes
  • DNA, Neoplasm / analysis
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / isolation & purification
  • ErbB Receptors / genetics*
  • Gene Amplification / genetics*
  • Gene Expression Regulation, Neoplastic / genetics*
  • Genes*
  • Humans
  • Kidney Neoplasms / genetics*
  • Nucleic Acid Hybridization
  • RNA, Neoplasm / analysis
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / isolation & purification

Substances

  • DNA Probes
  • DNA, Neoplasm
  • RNA, Neoplasm
  • ErbB Receptors