A core filamentation response network in Candida albicans is restricted to eight genes

PLoS One. 2013;8(3):e58613. doi: 10.1371/journal.pone.0058613. Epub 2013 Mar 14.

Abstract

Although morphological plasticity is a central virulence trait of Candida albicans, the number of filament-associated genes and the interplay of mechanisms regulating their expression remain unknown. By correlation-based network modeling of the transcriptional response to different defined external stimuli for morphogenesis we identified a set of eight genes with highly correlated expression patterns, forming a core filamentation response. This group of genes included ALS3, ECE1, HGT2, HWP1, IHD1 and RBT1 which are known or supposed to encode for cell- wall associated proteins as well as the Rac1 guanine nucleotide exchange factor encoding gene DCK1 and the unknown function open reading frame orf19.2457. The validity of network modeling was confirmed using a dataset of advanced complexity that describes the transcriptional response of C. albicans during epithelial invasion as well as comparing our results with other previously published transcriptome studies. Although the set of core filamentation response genes was quite small, several transcriptional regulators are involved in the control of their expression, depending on the environmental condition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / cytology*
  • Candida albicans / genetics*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal / genetics*
  • Humans
  • Hyphae / cytology
  • Hyphae / genetics
  • Models, Genetic
  • Transcription, Genetic
  • Transcriptome

Grant support

This work was financed by the German Ministry for Education and Science in the program Unternehmen Region (BMBF 03Z2JN21 to OK). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.