Serovar diversity of pathogenic Leptospira circulating in the French West Indies

PLoS Negl Trop Dis. 2013;7(3):e2114. doi: 10.1371/journal.pntd.0002114. Epub 2013 Mar 14.

Abstract

Background: Leptospirosis is one of the most important neglected tropical bacterial diseases in Latin America and the Caribbean. However, very little is known about the circulating etiological agents of leptospirosis in this region. In this study, we describe the serological and molecular features of leptospires isolated from 104 leptospirosis patients in Guadeloupe (n = 85) and Martinique (n = 19) and six rats captured in Guadeloupe, between 2004 and 2012.

Methods and findings: Strains were studied by serogrouping, PFGE, MLVA, and sequencing 16SrRNA and secY. DNA extracts from blood samples collected from 36 patients in Martinique were also used for molecular typing of leptospires via PCR. Phylogenetic analyses revealed thirteen different genotypes clustered into five main clades that corresponded to the species: L. interrogans, L. kirschneri, L. borgpetersenii, L. noguchi, and L. santarosai. We also identified L. kmetyi in at least two patients with acute leptospirosis. This is the first time, to our knowledge, that this species has been identified in humans. The most prevalent genotypes were associated with L. interrogans serovars Icterohaemorrhagiae and Copenhageni, L. kirschneri serovar Bogvere, and L. borgpetersenii serovar Arborea. We were unable to identify nine strains at the serovar level and comparison of genotyping results to the MLST database revealed new secY alleles.

Conclusions: The overall serovar distribution in the French West Indies was unique compared to the neighboring islands. Typing of leptospiral isolates also suggested the existence of previously undescribed serovars.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Genotype
  • Guadeloupe / epidemiology
  • Humans
  • Leptospira interrogans / classification*
  • Leptospira interrogans / genetics
  • Leptospira interrogans / immunology
  • Leptospira interrogans / isolation & purification
  • Leptospirosis / epidemiology*
  • Leptospirosis / microbiology*
  • Leptospirosis / veterinary*
  • Martinique / epidemiology
  • Molecular Sequence Data
  • Molecular Typing
  • Phylogeny
  • Polymerase Chain Reaction
  • Rats
  • Sequence Analysis, DNA
  • Serotyping

Substances

  • DNA, Bacterial

Associated data

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Grants and funding

This work was funded by the Institut Pasteur and the French Ministry of Health (InVS and CIRE Antilles-Guyane). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.