Effective separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide by altering conditions of polyacrylamide gel electrophoresis

J Immunol Methods. 1990 May 25;129(2):207-10. doi: 10.1016/0022-1759(90)90440-7.

Abstract

A method is described for the separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide 30:0.8, ratio = 37.5. A higher ratio of monomer to cross-linker, ratio = 172.4 used in a 15% acrylamide solution, readily separates the two peptide chains. This method facilitates the detection of the 52 kDa SSA/Ro component which otherwise might have been incorrectly assigned as a 48 kDa SSB/La polypeptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantigens / analysis*
  • Autoantigens / isolation & purification
  • Electrophoresis, Polyacrylamide Gel
  • Immunoblotting
  • Molecular Weight
  • RNA, Small Cytoplasmic*
  • Ribonucleoproteins*

Substances

  • Autoantigens
  • RNA, Small Cytoplasmic
  • Ribonucleoproteins
  • SS-A antigen
  • SS-B antigen
  • TROVE2 protein, human