As there has been little research into the epidemiological status of bovine neosporosis in Algeria, the main aims of this study were to confirm the circulation of Neospora caninum in the country and to determine its seroprevalence in dairy cattle in five provinces of Algeria's central northern region by means of enzyme-linked immunosorbent assay and indirect immunofluorescence. A serological study of 186 dairy cattle declared brucellosis-free established an overall seroprevalence of 12.37%, confirming that N. caninum is circulating in dairy cattle and underlining the need to introduce systematic serological screening for neosporosis to limit its spread and prevent it from being perpetuated on Algerian farms.