A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

Nat Methods. 2013 May;10(5):407-9. doi: 10.1038/nmeth.2413. Epub 2013 Mar 24.


We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.

MeSH terms

  • Animals
  • Chordata / metabolism*
  • Green Fluorescent Proteins / metabolism*
  • Molecular Sequence Data
  • Stochastic Processes


  • Green Fluorescent Proteins

Associated data

  • GENBANK/KC295282