Lack of response of murine peritoneal macrophages to in vitro activation by muramyl dipeptide (MDP). I. Macrophage activation by MDP is species dependent

Microbiol Immunol. 1990;34(3):323-35. doi: 10.1111/j.1348-0421.1990.tb01013.x.


Peritoneal exudate macrophages from mice, rats, and guinea pigs were assessed using six different parameters of macrophage activation to determine whether the cells were stimulated under similar experimental conditions. Peritoneal exudate macrophages from mice, irrespective of strain, were far less responsive to a variety of in vitro stimulatory effects of N-acetylmuramyl-L-alanyl-D-isoglutamine than those from rats or guinea pigs, while no significant differences were noted with the reactivity to stimulation by endotoxic lipopolysaccharide. We conclude that macrophage activation by MDP in vitro is species dependent.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylmuramyl-Alanyl-Isoglutamine / pharmacology*
  • Animals
  • Cells, Cultured
  • Cytotoxicity, Immunologic
  • Female
  • Glucosamine / metabolism
  • Guinea Pigs
  • In Vitro Techniques
  • Interleukin-1 / metabolism
  • Lipopolysaccharides / pharmacology
  • Macrophage Activation / drug effects*
  • Macrophage Activation / immunology
  • Macrophages / immunology*
  • Mice
  • Mice, Inbred Strains
  • Peritoneal Cavity / cytology*
  • Rats
  • Species Specificity
  • Tumor Necrosis Factor-alpha / metabolism


  • Interleukin-1
  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • Acetylmuramyl-Alanyl-Isoglutamine
  • Glucosamine