Molecular characterization of a new alphasatellite associated with a cassava mosaic geminivirus in Madagascar

Arch Virol. 2013 Aug;158(8):1829-32. doi: 10.1007/s00705-013-1664-0. Epub 2013 Mar 23.

Abstract

Two complete nucleotide sequences of an alphasatellite isolated from a cassava plant with mosaic disease symptoms in Madagascar are described and analyzed. While the helper begomovirus was identified as an isolate of East African cassava mosaic Kenya virus (EACMKV), its associated alphasatellite was most closely related (80 % nucleotide sequence identity) to cotton leaf curl Gezira alphasatellite. These satellite molecules have typical features of alphasatellites, with a single gene in the virion sense, an A-rich region and a stem-loop structure. According to the proposed species demarcation threshold of alphasatellites (83 % nucleotide identity), they are isolates of a new species for which we propose the name "Cassava mosaic alphasatellite".

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Begomovirus / genetics
  • DNA, Satellite / genetics*
  • DNA, Satellite / isolation & purification
  • Madagascar
  • Manihot / virology
  • Molecular Sequence Data
  • Phylogeny
  • Plant Diseases / virology
  • Sequence Analysis, DNA
  • Sequence Homology

Substances

  • DNA, Satellite

Associated data

  • GENBANK/HE984148
  • GENBANK/HE984149
  • RefSeq/NC_018628