Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex

Mol Nutr Food Res. 2013 Jun;57(6):1100-9. doi: 10.1002/mnfr.201200433. Epub 2013 Mar 25.


Scope: Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients.

Methods and results: The molecule, termed Man e5, was expressed in E. coli, characterized by amino acid analysis, mass spectrometry, circular dichroism, HPLC, and dynamic light scattering. A tertiary structural model of the protein was produced using bioinformatics and susceptibility to pepsin digestion was analyzed in vitro. Based on its high content of charged residues, heat stability, flexibility and lack of secondary structure elements, the allergen was determined a member of the intrinsically disordered protein family. Brazilian patients were selected based on manioc allergy and Italians based on latex allergy and sensitization to Hev b 5.71% of Brazilians and 40% of Italians were in vitro IgE positive to Man e5. Cross-inhibition assays suggest a possible involvement of this allergen in the latex-fruit syndrome.

Conclusion: Man e5, the first purified allergen from manioc demonstrates IgE cross-reactivity with Hev b 5. Data suggest Hev b 5 might act as primary sensitizer and could therefore lead to allergic manifestations upon manioc consumption without prior exposition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Allergens / genetics*
  • Allergens / immunology
  • Amino Acid Sequence
  • Antigens, Plant / genetics*
  • Antigens, Plant / immunology*
  • Brazil
  • Circular Dichroism
  • Cloning, Molecular
  • Cross Reactions / immunology
  • Female
  • Food Hypersensitivity / blood
  • Food Hypersensitivity / diagnosis
  • Food Hypersensitivity / immunology*
  • Humans
  • Immune Sera
  • Immunoglobulin E / immunology
  • Latex / immunology
  • Latex Hypersensitivity / blood
  • Latex Hypersensitivity / immunology
  • Male
  • Manihot / chemistry*
  • Manihot / immunology
  • Middle Aged
  • Models, Molecular
  • Molecular Sequence Data
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / immunology*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology


  • Allergens
  • Antigens, Plant
  • Immune Sera
  • Latex
  • Man e 5 allergen, Manihot esculenta
  • Plant Proteins
  • Recombinant Proteins
  • allergen Hev b 5
  • Immunoglobulin E

Associated data

  • GENBANK/JF710639