Recording of ion channel activity in planar lipid bilayer experiments

Methods Mol Biol. 2013;998:109-18. doi: 10.1007/978-1-62703-351-0_8.

Abstract

Planar lipid bilayer is an electrophysiological technique that enables study of functional activities of ion channels, porins, and other pore-forming molecular complexes. The main purpose of this method is to monitor ion channels' behavior at the single molecule level in the artificial membranes. Here, I describe the details of this technique that will underline formation of the lipid bilayers and incorporation and activation of the ion channel protein.

MeSH terms

  • Cytological Techniques / methods*
  • Glass / chemistry
  • Humans
  • Lipid Bilayers / metabolism*
  • TRPM Cation Channels / metabolism
  • Temperature

Substances

  • Lipid Bilayers
  • TRPM Cation Channels
  • TRPM8 protein, human