Comparing biocompatibility of gingival fibroblasts and bacterial strains on a different modified titanium discs

J Biomed Mater Res A. 2013 Oct;101(10):2915-24. doi: 10.1002/jbm.a.34598. Epub 2013 Mar 25.


The modification of implant surface situated in the area of peri-implant sulcus has important role in bacterial and cell adhesion. Six different chemically and physically modified titanium discs were prepared: glazed (Tis-MALP), unglazed (Tis-O), unglazed and alkali-etched (Tis-OA), unglazed and coated with ZrN (Tis-OZ), unglazed, sand blasted, and acid etched (Tis-OPAE), and unglazed, sand blasted, acid, and alkali etched (Tis-OPAAE). Analysis of surface topography was determined using scanning electron microscopy and atomic force microscopy (AFM). Biocompatibility of gingival fibroblasts was characterized by the production of tumor necrosis factor alpha, collagen I, matrix metalloproteinase 2 (MMP-2) after 24 and 72 h and expression of α3 β1 integrin and vinculin using enzyme-linked immunosorbent assay (ELISA) or modified ELISA after 6 and 24 h. Microorganism adhesion (five bacterial strains) and biofilm formation was also evaluated. The adhesion of bacteria and gingival fibroblasts was significantly higher on titanium disc Tis-OPAAE and biofilm formation on the same surface for Streptococcus mutans, Streptococcus gordonii, and Streptococcus intermedius. The gingival fibroblasts on Tis-OPAAE disc had also significantly lower production of MMP-2. The collagen production was significantly lower on all surfaces with roughness higher than 0.2 μm. This study confirmed that the titanium disc with the surface roughness 3.39 μm (Tis-OPAAE) supported the adhesion of bacterial strains as well as gingival fibroblasts.

Keywords: adhesion; bacterial strains; gingival fibroblasts; titanium modification.

Publication types

  • Comparative Study

MeSH terms

  • Bacterial Adhesion / drug effects
  • Biocompatible Materials / pharmacology*
  • Biofilms / drug effects
  • Biofilms / growth & development
  • Cell Adhesion / drug effects
  • Collagen Type I / biosynthesis
  • Dental Implants / microbiology
  • Fibroblasts / cytology*
  • Fibroblasts / drug effects
  • Fibroblasts / microbiology*
  • Focal Adhesions / drug effects
  • Focal Adhesions / metabolism
  • Gingiva / cytology*
  • Humans
  • Integrin alpha3beta1 / metabolism
  • Materials Testing*
  • Matrix Metalloproteinase 2 / biosynthesis
  • Microscopy, Atomic Force
  • Microscopy, Electron, Scanning
  • Spectrometry, X-Ray Emission
  • Streptococcus / cytology*
  • Streptococcus / drug effects
  • Streptococcus / physiology
  • Titanium / pharmacology*
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Vinculin / metabolism


  • Biocompatible Materials
  • Collagen Type I
  • Dental Implants
  • Integrin alpha3beta1
  • Tumor Necrosis Factor-alpha
  • Vinculin
  • Titanium
  • Matrix Metalloproteinase 2