Inhibition of 12-lipoxygenase reduces platelet activation and prevents their mitogenic function

Platelets. 2014;25(2):111-7. doi: 10.3109/09537104.2013.783688. Epub 2013 Mar 27.


The aim of the present study was to investigate the role of 12-lipoxygenase (12-LOX) on platelet-induced airway smooth muscle cell (ASMC) proliferation. Co-incubation of platelets and ASMC caused platelet activation as determined by morphological changes. Simultaneously, reactive oxygen species (ROS)-generation was detected and ASMC proliferation (measured by using the MTS assay) increased significantly. Furthermore, we found that the 12-LOX inhibitors cinnamyl-3,4-dihydroxy-α-cyanocinnamate (CDC) and Baicalein prevented platelet activation in a co-cultures of platelets and ASMC. The inhibitory effect of CDC and Baicalein on platelets was also registered in a pure platelet preparation. Specifically, the 12-LOX inhibitors reduced collagen-induced platelet aggregation both in the presence and absence of external added fibrinogen. Importantly, platelet-induced ASMC proliferation and ROS production generated during the platelet/ASMC interaction was significantly inhibited in the presence of 12-LOX inhibitors. In conclusion, our findings reveal that 12-LOX is crucial for the observed enhancement of ASMC proliferation in co-cultures of platelets and ASMC. The present result suggests that 12-LOX activity is important in the initial step of platelet/ASMC interaction and platelet activation. Such action of 12-LOX represents a potential important mechanism that may contribute to platelet-induced airway remodelling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Airway Remodeling
  • Arachidonate 12-Lipoxygenase / metabolism*
  • Blood Platelets / drug effects*
  • Blood Platelets / metabolism*
  • Cell Proliferation
  • Cells, Cultured
  • Coculture Techniques
  • Enzyme Activation
  • Humans
  • Lipoxygenase Inhibitors / pharmacology*
  • Myocytes, Smooth Muscle / metabolism
  • Platelet Activation / drug effects*
  • Reactive Oxygen Species / metabolism


  • Lipoxygenase Inhibitors
  • Reactive Oxygen Species
  • Arachidonate 12-Lipoxygenase