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. 2013 Mar 28;8(1):146.
doi: 10.1186/1556-276X-8-146.

Silica Nanoparticles as a Tool for Fluorescence Collection Efficiency Enhancement

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Free PMC article

Silica Nanoparticles as a Tool for Fluorescence Collection Efficiency Enhancement

Bartosz Krajnik et al. Nanoscale Res Lett. .
Free PMC article

Abstract

In this work we demonstrate enhancement of the fluorescence collection efficiency for chlorophyll-containing photosynthetic complexes deposited on SiO2 spherical nanoparticles. Microscopic images of fluorescence emission reveal ring-like emission patterns associated with chlorophyll-containing complexes coupled to electromagnetic modes within the silica nanoparticles. The interaction leaves no effect upon the emission spectra of the complexes, and the transient behavior of the fluorescence also remains unchanged, which indicates no influence of the silica nanoparticles on the radiative properties of the fluorophores. We interpret this enhancement as a result of efficient scattering of electromagnetic field by the dielectric nanoparticles that increases collection efficiency of fluorescence emission.

Figures

Figure 1
Figure 1
Scanning electron microscopy image and optical spectra of the silica nanoparticles. (a) Scanning electron microscopy image of the silica nanoparticles with a diameter of 1,100 nm. (b) Optical spectra of silica nanoparticles with diameters of 600 nm (dash-dot) and 1,100 nm (dash) compared to absorption spectrum of the PCP complex solution (solid) as well as its fluorescence (dot).
Figure 2
Figure 2
Wide-field fluorescence image of the PCP complexes on 1.1-μm-diameter silica nanoparticles and their fluorescence intensity. (a) Wide-field fluorescence image of the PCP complexes deposited on silica nanoparticles with a diameter of 1.1 μm. Excitation wavelength was 480 nm. (b) Histogram of the fluorescence intensity calculated from the wide-field fluorescence image. (c) Cross section of the fluorescence intensity obtained for the three nanoparticles shown in Figure 2a.
Figure 3
Figure 3
Wide-field fluorescence image of the PCP complexes on 0.6-μm-diameter silica nanoparticles and their fluorescence intensity. (a) Wide-field fluorescence image of the PCP complexes deposited on silica nanoparticles with a diameter of 0.6 μm. Excitation wavelength was 480 nm. (b) Histogram of the fluorescence intensity calculated from the wide-field fluorescence image. (c) Cross section of the fluorescence intensity obtained along the black line 1 for the three nanoparticles shown in Figure 3a.
Figure 4
Figure 4
Emission spectra and fluorescence decay curves of the PCP complexes. (a) Emission spectra of the PCP complexes deposited on (red) and off (black) silica nanoparticles. (b) Fluorescence decay curves of PCP deposited on (red) and off (black) silica nanoparticles. The excitation wavelength for both experiments was 480 nm. The transients are normalized, and the one measured for the PCP complexes off the silica nanoparticles was shifted vertically (multiplied by 10) for clarity.

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