Abstract
Current immuno-PCR methods either use bare or nanostructured DNA as a reporter or combine phage display for antigen binding and reporting, however, they are often complex to carry out and lack universality. We present a novel and universal design of immuno-PCR termed natural phage nanoparticle-mediated real-time immuno-PCR.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / immunology
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Bacteriophage T7 / genetics
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Bacteriophage T7 / metabolism*
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DNA, Viral / metabolism
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Fluorescein-5-isothiocyanate / chemistry
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Hepatitis B Surface Antigens / blood
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Hepatitis B Surface Antigens / immunology
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Humans
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Microscopy, Electron, Transmission
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Nanoparticles / chemistry*
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Proteins / analysis*
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Proteins / immunology
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Real-Time Polymerase Chain Reaction*
Substances
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Antibodies, Monoclonal
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DNA, Viral
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Hepatitis B Surface Antigens
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Proteins
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Fluorescein-5-isothiocyanate