Natural phage nanoparticle-mediated real-time immuno-PCR for ultrasensitive detection of protein marker

Chem Commun (Camb). 2013 May 8;49(36):3778-80. doi: 10.1039/c3cc40688a. Epub 2013 Mar 28.

Abstract

Current immuno-PCR methods either use bare or nanostructured DNA as a reporter or combine phage display for antigen binding and reporting, however, they are often complex to carry out and lack universality. We present a novel and universal design of immuno-PCR termed natural phage nanoparticle-mediated real-time immuno-PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Bacteriophage T7 / genetics
  • Bacteriophage T7 / metabolism*
  • DNA, Viral / metabolism
  • Fluorescein-5-isothiocyanate / chemistry
  • Hepatitis B Surface Antigens / blood
  • Hepatitis B Surface Antigens / immunology
  • Humans
  • Microscopy, Electron, Transmission
  • Nanoparticles / chemistry*
  • Proteins / analysis*
  • Proteins / immunology
  • Real-Time Polymerase Chain Reaction*

Substances

  • Antibodies, Monoclonal
  • DNA, Viral
  • Hepatitis B Surface Antigens
  • Proteins
  • Fluorescein-5-isothiocyanate