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. 2013 Jul;57:126-31.
doi: 10.1016/j.fct.2013.03.017. Epub 2013 Mar 26.

Anti-inflammatory Effect of Essential Oil and Its Constituents From Fingered Citron (Citrus Medica L. Var. Sarcodactylis) Through Blocking JNK, ERK and NF-κB Signaling Pathways in LPS-activated RAW 264.7 Cells

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Anti-inflammatory Effect of Essential Oil and Its Constituents From Fingered Citron (Citrus Medica L. Var. Sarcodactylis) Through Blocking JNK, ERK and NF-κB Signaling Pathways in LPS-activated RAW 264.7 Cells

Kil-Nam Kim et al. Food Chem Toxicol. .

Abstract

We investigated the composition of essential oil from fingered citron (Citrus medica L. var. sarcodactylis) (FCEO) peels by GC-MS and its anti-inflammatory effects on lipopolysaccharide (LPS) - stimulated mouse macrophage (RAW 264.7) cells. Fifteen compounds, representing 98.97% of the essential oil, were tentatively identified; the main constituents were limonene (52.44%) and γ-terpinene (28.41%). FCEO significantly inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) by suppressing the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, respectively. Additionally, FCEO suppressed the production of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6. FCEO attenuated LPS-induced nuclear factor-κB (NF-κB) activation via inhibition of inhibitor κB-α phosphorylation. Furthermore, FCEO blocked activation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) but not that of p38 mitogen-activated protein kinase. These results indicate that FCEO inhibits LPS-stimulated inflammation by blocking the NF-κB, JNK, and ERK pathways in macrophages, and demonstrate that FCEO possesses anti-inflammatory properties.

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