The decapping scavenger enzyme DCS-1 controls microRNA levels in Caenorhabditis elegans

Mol Cell. 2013 Apr 25;50(2):281-7. doi: 10.1016/j.molcel.2013.02.023. Epub 2013 Mar 28.

Abstract

In metazoans, microRNAs play a critical role in the posttranscriptional regulation of genes required for cell proliferation and differentiation. MicroRNAs themselves are regulated by a multitude of mechanisms influencing their transcription and posttranscriptional maturation. However, there is only sparse knowledge on pathways regulating the mature, functional form of microRNA. Here, we uncover the implication of the decapping scavenger protein DCS-1 in the control of microRNA turnover. In Caenorhabditis elegans, mutations in dcs-1 increase the levels of functional microRNAs. We demonstrate that DCS-1 interacts with the exonuclease XRN-1 to promote microRNA degradation in an independent manner from its known decapping scavenger activity, establishing two molecular functions for DCS-1. Our findings thus indicate that DCS-1 is part of a degradation complex that performs microRNA turnover in animals.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / enzymology*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Exoribonucleases / metabolism
  • Gene Expression
  • MicroRNAs / metabolism*
  • Mutation
  • N-Glycosyl Hydrolases / genetics
  • N-Glycosyl Hydrolases / metabolism*
  • Pyrophosphatases
  • RNA Interference
  • RNA Stability
  • RNA, Helminth / metabolism*
  • RNA-Induced Silencing Complex / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • MicroRNAs
  • RNA, Helminth
  • RNA-Induced Silencing Complex
  • Exoribonucleases
  • XRN-1 protein, C elegans
  • N-Glycosyl Hydrolases
  • Pyrophosphatases
  • dcs-1 protein, C elegans