Structure and antiviral activity of arabinogalactan with (1→6)-β-D-galactan core from Stevia rebaudiana leaves

Carbohydr Polym. 2013 Apr 15;94(1):179-84. doi: 10.1016/j.carbpol.2012.12.068. Epub 2013 Jan 4.


Cell wall polysaccharides from leaves of Stevia rebaudiana were extracted successively with water and with aq. 10% KOH. After the purification steps, homogeneous fractions (SFW-10RM and SSFK-10RM) were analyzed by sugar composition, HPSEC, methylation and (13)C NMR spectroscopy analysis. The results showed that SFW-10RM is a pectic arabinogalactan with an unusual β-(1→6)-linked D-Galp residues forming the main chain. Approximately 38% of the β-D-Galp units of the backbone carry branches on position O-3, consisting of single D-Galp units or arabinan side chains. Arabinose residues were found to occupy mostly the terminal positions in both furanose and pyranose forms and as 2-, 5- and 3,5-linked residues in these side chains. Fraction SSFK-10RM is a similar arabinogalactan, differing mainly in the relative proportions of arabinans attached to the galactan core and in the content of D-GalpA residues present in the pectic domain. The crude aqueous and alkaline extracts and homogeneous SSFK-10RM showed antiviral activity against Herpes Simplex Virus type-1 (HSV-1) in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / chemistry
  • Antiviral Agents / isolation & purification
  • Antiviral Agents / pharmacology*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chlorocebus aethiops
  • Galactans / chemistry
  • Galactans / isolation & purification
  • Galactans / pharmacology*
  • Herpesvirus 1, Human / drug effects*
  • Hydrophobic and Hydrophilic Interactions
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry*
  • Stevia / chemistry*
  • Vero Cells


  • Antiviral Agents
  • Galactans
  • Plant Extracts
  • 1,6-galactan
  • arabinogalactan