In this study, the complete mitogenome sequence of Chinese sucker Myxocyprinus asiaticus (Cypriniformes, Catostomidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,636 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes, and a noncoding control region (CR). CR of 944 bp length is located between tRNA(Pro) and tRNA(Phe). The overall base composition of M. asiaticus is 28.62% for A, 27.90% for C, 24.8% for T, and 18.70% for G, with a slight AT bias of 53.40%. The complete mitogenome may provide important DNA molecular data for further phylogenetic analyses for higher taxa of teleost fishes especially for the members of Cypriniformes.