CD30L/CD30 is critical for maintenance of IL-17A-producing γδ T cells bearing Vγ6 in mucosa-associated tissues in mice

Mucosal Immunol. 2013 Nov;6(6):1191-201. doi: 10.1038/mi.2013.18. Epub 2013 Apr 3.


CD30 ligand (CD30L, CD153), a member of the tumor necrosis factor (TNF) superfamily, and its receptor CD30 are important for differentiation and activation of CD4(+) T helper type 17 (Th17) cells. In this report, we demonstrate that the interleukin 17A (IL-17A)-producing γδ T cells normally developed in the fetal thymus, whereas Vγ1(-)Vγ4(-) γδ T cells expressed Vγ6/Vδ1 gene transcript selectively decreased in mucosa-associated tissues in naive CD30KO or CD30LKO mice. Moreover, CD30 and CD30L were expressed preferentially by Vγ1(-)Vγ4(-) γδ T cells in naive mice. The bacteria clearance was attenuated by the impaired response of the IL-17A-producing γδ T cells and decreased infiltration of neutrophils in CD30KO or CD30LKO mice. In vivo administration of agonistic anti-CD30 monoclonal antibody restored the ability of protection against Listeria monocytogenes by enhancing Vγ1(-)Vγ4(-) γδ T cells producing IL-17A not only in wild-type but also CD30LKO mice. Taken together, it appears that CD30L/CD30 signaling plays an important role in the maintenance and activation of IL-17A-producing γδ T cells presumably bearing Vγ6 in the mucosa-associated tissues of mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / administration & dosage
  • Antibodies, Monoclonal / pharmacology
  • CD30 Ligand / genetics
  • CD30 Ligand / metabolism*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Homeostasis / drug effects
  • Interleukin-17 / metabolism
  • Ki-1 Antigen / agonists
  • Ki-1 Antigen / genetics
  • Ki-1 Antigen / metabolism*
  • Listeriosis / immunology*
  • Lymphocyte Activation
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mucous Membrane / immunology*
  • Mucous Membrane / microbiology
  • Receptors, Antigen, T-Cell, gamma-delta / metabolism
  • Th17 Cells / drug effects
  • Th17 Cells / immunology*


  • Antibodies, Monoclonal
  • CD30 Ligand
  • Interleukin-17
  • Ki-1 Antigen
  • Receptors, Antigen, T-Cell, gamma-delta
  • T-cell receptor Vgamma6, mouse
  • Tnfsf8 protein, mouse