Structure of a classical broadly neutralizing stem antibody in complex with a pandemic H2 influenza virus hemagglutinin

J Virol. 2013 Jun;87(12):7149-54. doi: 10.1128/JVI.02975-12. Epub 2013 Apr 3.

Abstract

We report the structural characterization of the first antibody identified to cross-neutralize multiple subtypes of influenza A viruses. The crystal structure of mouse antibody C179 bound to the pandemic 1957 H2N2 hemagglutinin (HA) reveals that it targets an epitope on the HA stem similar to those targeted by the recently identified human broadly neutralizing antibodies. C179 also inhibits the low-pH conformational change of the HA but uses a different angle of approach and both heavy and light chains.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / chemistry*
  • Antibodies, Monoclonal / immunology
  • Antibodies, Neutralizing / chemistry*
  • Antibodies, Neutralizing / immunology
  • Antibodies, Viral / chemistry*
  • Antibodies, Viral / immunology
  • Cross Reactions
  • Crystallography, X-Ray
  • Epitopes / chemistry
  • Epitopes / immunology
  • Hemagglutinin Glycoproteins, Influenza Virus / immunology*
  • Humans
  • Influenza A Virus, H2N2 Subtype / immunology*
  • Influenza A virus / classification
  • Influenza A virus / immunology*
  • Influenza, Human / immunology
  • Influenza, Human / virology
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Neutralization Tests
  • Pandemics

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Epitopes
  • Hemagglutinin Glycoproteins, Influenza Virus

Associated data

  • PDB/4HLZ