Yerba mate tea and mate saponins prevented azoxymethane-induced inflammation of rat colon through suppression of NF-κB p65ser(311) signaling via IκB-α and GSK-3β reduced phosphorylation

Biofactors. Jul-Aug 2013;39(4):430-40. doi: 10.1002/biof.1083. Epub 2013 Mar 29.

Abstract

Yerba mate tea (YMT) has a chemopreventive role in a variety of inflammatory diseases. The objective was to determine the capability of YMT and mate saponins to prevent azoxymethane (AOM)-induced colonic inflammation in rats. YMT (2% dry leaves, w/v, as a source of drinking fluid) (n = 15) and mate saponins (0.01% in the diet, at a concentration present in one cup of YMT) (n = 15) were given ad libitum to rats 2 weeks prior to AOM-injection until the end of the study; while control rats (n = 15) received a basal diet and drinking water. After 8-weeks of study, total colonic mucosa was scraped (n = 3 rats/group) and the remaining colons (n =12 rats/group) were cut into three equal sections and aberrant crypt foci (ACF) were analyzed. YMT reduced ACF formation from 113 (control group) to 89 (P < 0.05). YMT and mate saponins reduced the expression of proinflammatory molecules COX-2 and iNOS with concomitant reduction in p-p65 (P < 0.05). Immunohistochemical analysis of the formalin-fixed middle colons showed that YMT and mate saponins reduced the expression of p-p65(ser311) by 45.7% and 43.1%, respectively, in comparison to the control (P < 0.05). In addition, the expression of molecules upstream of NF-κB such as p-IκB-α and p-GSK-3β(Y216) was downregulated by YMT 24.7% and 24.4%, respectively (P < 0.05). Results suggest the mechanism involved in the chemopreventive effect of YMT and mate saponin consumption in AOM induced-colonic inflammation in rats is through inhibition of NF-κB.

Keywords: NF-κB signaling; Yerba mate; colon cancer; inflammation; mate saponins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Anti-Inflammatory Agents / therapeutic use
  • Anticarcinogenic Agents / pharmacology
  • Anticarcinogenic Agents / therapeutic use
  • Azoxymethane
  • Beverages
  • Colitis / chemically induced
  • Colitis / prevention & control*
  • Colon / drug effects
  • Colon / immunology
  • Colon / pathology
  • Colorectal Neoplasms / prevention & control
  • Glycogen Synthase Kinase 3 / metabolism
  • Glycogen Synthase Kinase 3 beta
  • I-kappa B Proteins / metabolism
  • Ilex paraguariensis / chemistry*
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / immunology
  • Male
  • NF-KappaB Inhibitor alpha
  • Neoplasm Proteins / metabolism
  • Nucleocytoplasmic Transport Proteins / metabolism
  • Phosphorylation
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Proliferating Cell Nuclear Antigen / metabolism
  • Protein Processing, Post-Translational
  • Rats
  • Rats, Inbred F344
  • Saponins / pharmacology*
  • Saponins / therapeutic use
  • Signal Transduction*

Substances

  • Anti-Inflammatory Agents
  • Anticarcinogenic Agents
  • I-kappa B Proteins
  • Neoplasm Proteins
  • Nfkbia protein, rat
  • Nucleocytoplasmic Transport Proteins
  • Plant Extracts
  • Proliferating Cell Nuclear Antigen
  • Saponins
  • p65 oncofetal mRNA transport protein, rat
  • NF-KappaB Inhibitor alpha
  • Glycogen Synthase Kinase 3 beta
  • Gsk3b protein, rat
  • Glycogen Synthase Kinase 3
  • Azoxymethane