Separation of nonfucosylated antibodies with immobilized FcγRIII receptors

Biotechnol Prog. May-Jun 2013;29(3):825-8. doi: 10.1002/btpr.1717. Epub 2013 Apr 1.

Abstract

Post-translational modifications can dramatically impact protein activity, but identifying such structure:function relationships, as well as capitalizing on functionally enhanced variants, is a significant challenge. Here, affinity chromatography resins that contained immobilized FcγRIII receptors were used to enrich nonfucosylated antibodies 6- to 9-fold, offering what may be a tractable method for both the identification of post-translational modifications that affect function, as well as a means to enrich variants with enhanced activity.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / isolation & purification*
  • Antibodies, Monoclonal / metabolism
  • CHO Cells
  • Chromatography, Affinity / instrumentation
  • Chromatography, Affinity / methods*
  • Cricetinae
  • Cricetulus
  • Fucose
  • Humans
  • Immobilized Proteins / chemistry*
  • Immobilized Proteins / metabolism
  • Receptors, IgG / chemistry*
  • Receptors, IgG / metabolism

Substances

  • Antibodies, Monoclonal
  • Immobilized Proteins
  • Receptors, IgG
  • Fucose